The following information accompanied stocks donated to the Bloomington Stock Center by Larry Reiter, University of Tennessee Health Science Center.
P{UAS-Ube3a.R} expresses an untagged Ube3a protein under UAS control. As mentioned in Valdez et al. (2015; FBrf0228986), Hope et al. (2017; FBrf0237185) and Hope et al. (2019; FBrf0241588), the same LD21888 cDNA clone was used in its construction that was used to make a plasmid for cultured cells in Reiter et al. (2006; FBrf0192865), but the construction of P{UAS-Ube3a.R} was not described.
P{UAS-Ube3a.R}27 and P{UAS-Ube3a.R}45 are second chromosome insertions. As described in Valdez (2015; FBrf0228986), P{UAS-Ube3a.R}27 is expressed strongly under GAL4 activation, whereas P{UAS-Ube3a.R}45 is expressed at lower levels.
P{UAS-FLAG-Ube3a}51 is a homozygous viable and fertile, third chromosome insertion that expresses Ube3a protein with an N-terminal FLAG tag under UAS control. It was generated in the work described in Ferdousy et al. (2011; FBrf0212886).
P{UAS-FLAG-Ube3a} was used in Ferdousy et al. (2011; FBrf0212886) and Jensen et al. (2013; FBrf0221414). P{UAS-Ube3a.R} was used in Hope et al. (2017; FBrf0237185) and Hope et al. (2019; FBrf0241588). Both constructs were used in Valdez et al. (2015; FBrf0228986) where insertions can be distinguished by the "27", "45" and "51" identifiers.