FlyBase Allele Report: Dmel\Ube3a[UAS.Tag:FLAG]

General Information

Symbol

Dmel\Ube3a^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0256608

Feature type

allele

Associated gene

Dmel\Ube3a

Associated Insertion(s)

Carried in Construct

P{UAS-FLAG-Ube3a}

Also Known As

UAS-Dube3a

Key Links

Transgenic product class

wild_type

(Ferdousy et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ferdousy et al., 2011)

Mutagen

in vitro construct

(Ferdousy et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-FLAG-Ube3a}

Cytology

Description

UAS regulatory sequences drive expression of Ube3a coding sequences tagged with Tag:FLAG.

(Ferdousy et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Ube3a

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA

(Valdez et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^elav-C155

(Ferdousy et al., 2011)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Valdez et al., 2015)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav-C155

(Valdez et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Valdez et al., 2015)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Valdez et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Ferdousy et al., 2011)

eye, with Scer\GAL4^GMR.PU

(Valdez et al., 2015)

synaptic vesicle | third instar larval stage, with Scer\GAL4^elav-C155

(Valdez et al., 2015)

Detailed Description

Statement

Reference

At the neuromuscular junction, stimulations at 15Hz intermittently fail to evoke excitatory junction potentials (EJPs) in almost half of Ube3a^UAS.Tag:FLAG third instar larvae with over-expression driven by Scer\GAL4^elav-C155, significantly more failures than in controls. There are no significant differences in EJP amplitude over the course of 30 stimulations at 15Hz in larvae that do not exhibit failures. There is no significant difference between controls, Ube3a^UAS.Tag:FLAG in mEJP amplitude or frequency in the presence of tetrodotoxin (TTX). In the absence of TTX, there is a significant correlation between amplitude of spontaneous depolarizations seen during mEJP recordings and evoked EJPs in the same larva; the percentage of larvae with spontaneous depolarizations is significantly higher than controls in larvae with Ube3a^UAS.Tag:FLAG driven by Scer\GAL4^elav-C155.

There is no significant difference in active zone density, bouton numbers or synaptic vesicle number at the neuromuscular junction of third instar larvae with Ube3a^UAS.Tag:FLAG driven by Scer\GAL4^elav-C155, compared to wild type. Larvae with Ube3a^UAS.Tag:FLAG driven by Scer\GAL4^elav-C155 have significantly smaller vesicles than wild type. There are also significantly fewer active zones at the neuromuscular junctions of third instar larvae with Ube3a^UAS.Tag:FLAG driven by Scer\GAL4^elav-C155 (though these numbers could be artificially inflated).

Flies with Ube3a^UAS.Tag:FLAG driven by Scer\GAL4^GMR.PU have an intermediate rough eye phenotype.

(Valdez et al., 2015)

Overexpression of Ube3a^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^GMR.PF results in a rough eye phenotype.

Expression of Ube3a^{Scer\UAS.T:Zzzz\FLAG} in the nervous system under the control of Scer\GAL4^elav-C155 results in an increase in dopamine and a concurrent increase in locomotor activity.

(Ferdousy et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has eye phenotype, enhanceable by Pu^r1

(Ferdousy et al., 2011)

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has interommatidial bristle phenotype, enhanceable by Pu^r1

(Ferdousy et al., 2011)

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has eye phenotype, enhanceable by Pu^EY04414

(Ferdousy et al., 2011)

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has interommatidial bristle phenotype, enhanceable by Pu^EY04414

(Ferdousy et al., 2011)

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has eye phenotype, enhanceable by Pu^EY02616

(Ferdousy et al., 2011)

Scer\GAL4^GMR.PF, Ube3a^UAS.Tag:FLAG has interommatidial bristle phenotype, enhanceable by Pu^EY02616

(Ferdousy et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A Pu^r1, Pu^EY04414, or Pu^EY02616a heterozygous background enhances the Scer\GAL4^GMR.PF-> Ube3a^{Scer\UAS.T:Zzzz\FLAG} rough eye phenotype, causing a glazed appearance, loss of inter-ommatidial bristles and a yellowish discoloration, indicative of underlying neurodegeneration.

(Ferdousy et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90377

y¹ w^*; P{UAS-FLAG-Ube3a}51

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ube3a^{Scer\UAS.T:Zzzz\FLAG}

Ube3a^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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