The following information accompanied stocks donated to the Bloomington Stock Center by Benjamien Moeyaert and Eric Schreiter, Janelia Research Campus. 
CaMPARI2 is a new fluorescent protein-based calcium sensor converting from green to red fluorescence in the presence of elevated intracellular calcium and light exposure which was derived from the CaMPARI sensor protein (Fosque et al., 2015; FBrf0227755) by site-directed mutagenesis. CaMPARI2 has improved fluorescence brightness, faster kinetics of calcium unbinding and a lower photoconversion rate in low calcium conditions.
P{UAS-CaMPARI2} and PBac{UAS-CaMPARI2} are transgenes expressing CaMPARI2 tagged with a nuclear export signal and a His tag at the N-terminus and FLAG, HA and myc tags at the C-terminus under UAS control. They were constructed from insertion of the NES-His-CaMPARI2 sequence into the 10xUAS-IVS-Syn21-p10 vector described in Pfeiffer et al. (2012; FBrf0218136) and its subsequent insertion into attP target sites.
P{UAS-CaMPARI2}su(Hw)attP8 is an insertion into the P{CaryIP}su(Hw)attP8 target site at 8E10.
P{UAS-CaMPARI2}su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at 50F1.
PBac{UAS-CaMPARI2}VK00005 is an insertion into the PBac{y+-attP-9A}VK00005 target site at 75A10.
P{UAS-CaMPARI2.L398T} is identical to P{UAS-CaMPARI2} except for a sequence change substituting threonine for leucine at residue 398, which results in lower calcium affinity.
P{UAS-CaMPARI2.L398T}su(Hw)attP8 is an insertion into the P{CaryIP}su(Hw)attP8 target site at 8E10.
P{UAS-CaMPARI2.L398T}su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at 50F1.
P{LexAOp-CaMPARI2} is identical to P{UAS-CaMPARI2} except for the substitution of 13xLexAOp for 10xUAS. Likewise, PBac{LexAOp-CaMPARI2} is identical to PBac{UAS-CaMPARI2} except for the LexAOp substitution.
P{LexAOp-CaMPARI2}su(Hw)attP8 is an insertion into the P{CaryIP}su(Hw)attP8 target site at 8E10.
P{LexAOp-CaMPARI2}su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at 50F1.
PBac{LexAOp-CaMPARI2}VK00005 is an insertion into the PBac{y+-attP-9A}VK00005 target site at 75A10.
P{LexAOp-CaMPARI2.L398T} is identical to P{LexAOp-CaMPARI2} except for a sequence change substituting threonine for leucine at residue 398, which results in lower calcium affinity. Likewise, PBac{LexAOp-CaMPARI2.L398T} and PBac{LexAOp-CaMPARI2} are identical except for the L398T substitution.
P{LexAOp-CaMPARI2.L398T}su(Hw)attP8 is an insertion into the P{CaryIP}su(Hw)attP8 target site at 8E10.
P{LexAOp-CaMPARI2.L398T}su(Hw)attP5 is an insertion into the P{CaryIP}su(Hw)attP5 target site at 50F1.
PBac{LexAOp-CaMPARI2.L398T}VK00005 is an insertion into the PBac{y+-attP-9A}VK00005 target site at 75A10.