Isolation and characterization of Df(1)BSC868 Kim Cook, Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC868 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f06086 and P{XP}norpAd05473. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118 PBac{WH}f06086/w1118 P{XP}norpAd05473; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC868 from the segment of PBac{WH}f06086 to the left of its FRT site and the segment of P{XP}norpAd05473 to the right of its FRT site. The breakpoints of Df(1)BSC868 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are X:3825573-3825594 ;4218814-4218913 and the cytological breakpoints predicted from these coordinates are 3F7;4B6. It failed to complement brnfs.107. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.