Isolation and characterization of Df(2L)BSC862
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC862 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07564 and PBac{WH}f02298. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; P{XP}d07564/PBac{WH}f02298 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC862 from the segment of P{XP}d07564 to the left of its FRT site and the segment of PBac{WH}f02298 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(2L)BSC862 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:14490657 ;14755846 and the cytological breakpoints predicted from these coordinates are 35B2;35B5. Df(2L)BSC862 failed to complement nocSco and Df(2L)ED793.