From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(1)BSC627
Date: 	Mon, 29 Sep 2008  15:38:38  -0400  ( 20:38  BST)
Isolation and characterization of Df(1)BSC627
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC627 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG12659[f07899] and P{XP}CG12075[d09295]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of PBac{WH}CG12659[f07899]/P{XP}CG12075[d09295]; MKRS, 
P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC627 from the segment 
of PBac{WH}CG12659[f07899] to the left of its FRT site and the 
segment of P{XP}CG12075[d09295] to the right of its FRT site. Its 
presence was verified using the PCR methods and primers described in 
Parks et al. The cytological breakpoints of Df(1)BSC627 predicted 
from the Release 5 genomic coordinates of the transposable element 
insertions sites are 7F7;8B4. It failed to complement oc[1] and Lim[E9].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX