From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(1)BSC587
Date: 	Wed, 03 Sep 2008  11:58:40  -0400  ( 16:58  BST)
Isolation and characterization of Df(1)BSC587
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC587 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f06521 and P{XP}CG1532[d07742]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
PBac{WH}f06521/P{XP}CG1532[d07742]; MKRS, P{hsFLP}86E/+ females 
crossed to Binsinscy/Y males. These females were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC587 from the segment of 
PBac{WH}f06521 to the left of its FRT site and the segment of 
P{XP}CG1532[d07742] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(1)BSC587 predicted from the Release 
5 genomic coordinates of the transposable element insertions sites 
are 19A4;19E7. It failed to complement sw[1].