From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(1)BSC583
Date: 	Wed, 03 Sep 2008  11:58:39  -0400  ( 16:58  BST)
Isolation and characterization of Df(1)BSC583
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC583 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}baz[f07341] and P{XP}CG8316[d01774]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of PBac{WH}baz[f07341]/P{XP}CG8316[d01774]; MKRS, 
P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC583 from the segment 
of PBac{WH}baz[f07341] to the left of its FRT site and the segment of 
P{XP}CG8316[d01774] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(1)BSC583 predicted from the Release 
5 genomic coordinates of the transposable element insertions sites 
are 15F1;16B10. It failed to complement f[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX