From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(2R)BSC604
Date: 	Wed, 03 Sep 2008  12:00:30  -0400  ( 17:00  BST)
Isolation and characterization of Df(2R)BSC604
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC604 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}CG13585[d02774] and PBac{WH}f05560. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}CG13585[d02774]/PBac{WH}f05560 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/SM6a females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC604 from the segment 
of P{XP}CG13585[d02774] to the left of its FRT site and the segment 
of PBac{WH}f05560 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC604 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 60D4;60E11. Df(2R)BSC604 failed to complement pio[2R-16], 
Dll[5] and Eps-15[EP2513].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX