From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(1)BSC546
Date: 	Wed, 11 Jun 2008  14:30:22  -0400  ( 19:30  BST)
Isolation and characterization of Df(1)BSC546
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC546 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f07309 and P{XP}CG10990[d00598]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
PBac{WH}f07309/P{XP}CG10990[d00598]; MKRS, P{hsFLP}86E/+ females 
crossed to Binsinscy/Y males. These females were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC546 from the segment of 
PBac{WH}f07309 to the left of its FRT site and the segment of 
P{XP}CG10990[d00598] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(1)BSC546 predicted from the Release 
5 genomic coordinates of the transposable element insertions sites 
are 12B4;12B4. It failed to complement g[1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX