From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Stacey Christensen <sjchristXXXX>, Kim Cook <ruacookXXXX>, kaufman@XXXX
Subject: 	Isolation and characterization of Df(1)BSC538
Date: 	Wed, 11 Jun 2008  14:28:27  -0400  ( 19:28  BST)
Isolation and characterization of Df(1)BSC538
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC538 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f06347 and P{XP}d06864. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
PBac{WH}f06347/P{XP}d06864; MKRS, P{hsFLP}86E/+ females crossed to 
Binsinscy/Y males. These females were heat shocked as larvae as 
described in Parks et al., Nature Genetics 36: 288-292, 2004 
(FBrf0175003). This cross and crosses in preceding and succeeding 
generations maintained the original genetic background of the 
Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC538 from the segment of 
PBac{WH}f06347 to the left of its FRT site and the segment of 
P{XP}d06864 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(1)BSC538 predicted from the Release 5 
genomic coordinates of the transposable element insertions sites are 
8C17;8E4. It failed to complement Dsor1[G79] and lz[77a7].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX