From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3R)BSC519
Date: 	Tue, 15 Apr 2008  15:32:19  -0400  ( 20:32  BST)
Isolation and characterization of Df(3R)BSC519
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC519 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}tok[d00218] and PBac{WH}CG5807[f03761]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}tok[d00218]/PBac{WH}CG5807[f03761] males. The 
males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC519 from the segment of P{XP}tok[d00218] to the 
left of its FRT site and the segment of PBac{WH}CG5807[f03761] to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3R)BSC519 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
96A18;96A23. Df(3R)BSC519 failed to complement tld[B4].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX