From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC452
Date: 	Tue, 15 Apr 2008  14:43:32  -0400  ( 19:43  BST)
Isolation and characterization of Df(3L)BSC452
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC452 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d00582 and PBac{WH}Pka-R1[f03635]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d00582/PBac{WH}Pka-R1[f03635] males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC452 from the segment of 
P{XP}d00582 to the left of its FRT site and the segment of 
PBac{WH}Pka-R1[f03635] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC452 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 77E1;77F1. Df(3L)BSC452 failed to complement kni[ri-1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX