From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Cc: 	Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(3L)BSC448
Date: 	Tue, 15 Apr 2008  14:41:00  -0400  ( 19:41  BST)
Isolation and characterization of Df(3L)BSC448
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC448 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG32425[f01627] and P{XP}d06749. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}CG32425[f01627]/P{XP}d06749 males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC448 from the segment of 
PBac{WH}CG32425[f01627] to the left of its FRT site and the segment 
of P{XP}d06749 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC448 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 77C6;77E4. Df(3L)BSC448 failed to complement kni[ri-1].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX