Date: Mon, 29 Oct 2007  12:07:20  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC365
Isolation and characterization of Df(3L)BSC365
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC365 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d10752 and PBac{WH}CG9018[f03052]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d10752/PBac{WH}CG9018[f03052] males. These males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC365 from the segment of 
P{XP}d10752 to the left of its FRT site and the segment of 
PBac{WH}CG9018[f03052] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3L)BSC365 predicted from the 
Release 5 genomic coordinates of the transposable element insertion 
sites are 62B7;62D3. Df(3L)BSC365 failed to complement sls[1] and 
Df(3L)ED4284. Df(3L)FDD-0116595 is a synonym for Df(3L)BSC365.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX