Date: Mon, 29 Oct 2007  12:07:19  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC364
Isolation and characterization of Df(3L)BSC364
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC364 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}alpha-Spec[d03930] and 
PBac{WH}zormin[f00549].  The deletion was isolated as a chromosome 
lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] 
females crossed to P{hsFLP}1, y[1] w[1118]; 
P{XP}alpha-Spec[d03930]/PBac{WH}zormin[f00549] males. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC364 from the segment 
of P{XP}alpha-Spec[d03930] to the left of its FRT site and the 
segment of PBac{WH}zormin[f00549] to the right of its FRT site. Its 
presence was verified using the PCR methods and primers described in 
Parks et al. The cytological breakpoints of Df(3L)BSC364 predicted 
from the Release 5 genomic coordinates of the transposable element 
insertion sites are 62B4;62C4. Df(3L)BSC364 failed to complement 
sls[1] and Df(3L)ED4284. Df(3L)FDD-0053426 is a synonym for Df(3L)BSC364.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX