From: Kevin Cook <kcook@XXXX> To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX> Subject: Isolation and characterization of Df(2L)BSC354 Date: Mon, 29 Oct 2007 12:07:19 -0400 ( 16:07 GMT) Isolation and characterization of Df(2L)BSC354 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC354 was isolated as a FLP recombinase-induced recombination event involving P{XP}d11570 and PBac{WH}f07568. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}d11570/PBac{WH}f07568 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC354 from the segment of P{XP}d11570 to the left of its FRT site and the segment of PBac{WH}f07568 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC354 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 26D7;26E3. It failed to complement eya[cli-IID] and eya[2]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX