From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2L)BSC353
Date: 	Mon, 29 Oct 2007  12:07:19  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2L)BSC353
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC353 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}Gpdh[f02250] and P{XP}ade2[d06349]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
PBac{WH}Gpdh[f02250]/P{XP}ade2[d06349] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC353 from the segment of 
PBac{WH}Gpdh[f02250] to the left of its FRT site and the segment of 
P{XP}ade2[d06349] to the right of its FRT site. The cytological 
breakpoints of Df(2L)BSC353 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
26A3;26B3. It failed to complement Vm26Ab[QJ42] and chic[221]. 
Df(2L)FDD-0289332 is a synonym for Df(2L)BSC353.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX