Date: Thu, 22 Mar 2007  14:52:04  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC251
Isolation and characterization of Df(2L)BSC251
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC251 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}CG13130[d11066] and PBac{WH}f02264. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}CG13130[d11066]/PBac{WH}f02264 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC251 from the segment 
of P{XP}CG13130[d11066] to the left of its FRT site and the segment 
of PBac{WH}f02264 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2L)BSC251 predicted from the 
Release 4 genomic coordinates of the transposable element insertions 
sites are 30F5;31A1. It failed to complement bib[1].