Date: Tue, 29 Aug 2006 15:38:07 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC168 Cc: kaufmanXXXX, mdealXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX> Isolation and characterization of Df(2L)BSC168 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC168 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG11030[f07078] and P{XP}eIF-4a[d06487]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{WH}CG11030[f07078]/P{XP}eIF-4a[d06487] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC168 from the segment of PBac{WH}CG11030[f07078] to the left of its FRT site and the segment of P{XP}eIF-4a[d06487] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC168 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 25F4;26B2. It failed to complement Vm26Ab[QJ42] and chic[221]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX