Date: Wed, 30 Aug 2006  15:14:28  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC209
Isolation and characterization of Df(2L)BSC209
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC209 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d05552 and PBac{WH}pie[f05500]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d05552/PBac{WH}pie[f05500] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC209 from the segment 
of P{XP}d05552 to the left of its FRT site and the segment of 
PBac{WH}pie[f05500] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}d05552 to be at 
Release 3 genomic coordinate 10314218 on chromosome arm 2L, which is 
predicted to lie in 31D7 on both the Release 3 and Release 4 maps. 
PBac{WH}pie[f05500] is predicted to lie in 31D11 on the Release 4 
map. Consequently, the cytological breakpoints of Df(2L)BSC209 are 
predicted to be 31D7;31E1. It failed to complement cdc2[E1-23], 
da[1], RpS27A[mfs31] and RnrL[k06709].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX