Date: Mon, 11 Jul 2005  12:36:49  \-0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC108
Cc: jedeal@XXXX
Isolation and characterization of Df(2L)BSC108
Jennifer Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC108 was isolated as a FLP recombinase-induced recombination event
involving P{XP}d04594 and PBac{RB}e02576. The deletion was isolated as a
chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118;
P{XP}d04594/PBac{RB}e02576 males crossed to w1118; P{w+mC=hs-hid}2,
wgSp-1/CyO females. These males were heat shocked as larvae as described
in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This
cross and crosses in preceding and succeeding generations maintained the
original genetic background of the Exelixis insertion stocks (Thibault et
al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination
event generated the genetic element P+PBac{XP5.RB3}BSC108 from the segment
of P{XP}d04594 to the left of its FRT site and the segment of
PBac{RB}e02576 to the right of its FRT site. Its presence was verified
using the PCR methods and primers described in Parks et al with the
substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus
or RB3' minus primer in the Hybrid PCR protocol in the Supplementary
Methods. Exelixis, Inc. determined the insertion site of P{XP}d04594 to be
at Release 3 genomic coordinate 6866624 on chromosome arm 2L and the
insertion site of PBac{RB}e02576 to be at Release 3 genomic coordinate
6936961 on arm 2L. The Gene Disruption project determined the insertion
site of P{XP}d04594 to be at Release 3 genomic coordinate 6866623 on arm
2L. The cytological breakpoints of Df(2L)BSC108 predicted from these
coordinates are 27C2;27C6. It failed to complement
P{SUPor-P}CG10354KG04760, P{PZ}Hrb27C02647, P{SUPor-P}CG18304KG00684
and weeES1.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX