Date: Thu, 10 Mar 2005  11:37:49  \-0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC101
Cc: kaufmanXXXX, jedealXXXX, medeal@XXXX,
Rachel Andrade <randrade@XXXX>
Isolation and characterization of Df(2L)BSC101
Jennifer Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC101 was isolated as a FLP recombinase-induced recombination event
involving PBac{WH}f06575 and P{XP}d10944. The deletion was isolated as a
chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118;
PBac{WH}f06575/ P{XP}d10944 males crossed to w1118; P{w+mC=hs-hid}2,
wgSp-1/CyO females. These males were heat shocked as larvae as described
in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This
cross and crosses in preceding and succeeding generations maintained the
original genetic background of the Exelixis insertion stocks (Thibault et
al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination
event generated the genetic element P+PBac{XP5.WH5}BSC101 from the segment
of PBac{WH}f06575 to the left of its FRT site and the segment of
P{XP}d10944 to the right of its FRT site. Its presence was verified using
the PCR methods and primers described in Parks et al. Exelixis, Inc.
determined the insertion site of PBac{WH}f06575 to be at Release 3 genomic
coordinate 21599868 on chromosome arm 2L and the insertion site of
P{XP}d10944 to be at Release 3 genomic coordinate 21662045 on arm 2L. The
Gene Disruption project determined the insertion site of P{XP}d10944 to be
at Release 3 genomic coordinate 21662045 on arm 2L. The cytological
breakpoints of Df(2L)BSC101 predicted from these coordinates are
40A2;40A5. Df(2L)BSC101 failed to complement Df(2L)Exel6049.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-856-1213
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-7005 kcook@XXXX