Date: Wed, 12 May 2004 15:19:39 \-0500 To: rd120XXXX, flybase-updatesXXXX, Annette Parks <annettep@XXXX> From: Kevin Cook <kcook@XXXX> Subject: InR constructs and insertions The following information was provided by Annette Parks of Exelixis, Inc. In P{GMR-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to accession AAC47458 were cloned into P{Express-glass}. P{GMR-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{GMR-InR.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-InR.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{UAS-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to accession AAC47458 were cloned into P{Express-UAS}. P{UAS-InR.Exel}2 is a homozygous viable and fertile, second chromosome insertion. In P{sev-InR.Exel}, InR (FBgn0013984) coding sequences corresponding to accession AAC47458 were cloned into either P{Express-sev1x} or P{Express-sev3x}. P{sev-InR.Exel}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{sev-InR.Exel}2 is a homozygous viable and fertile, second chromosome insertion. P{sev-InR.Exel}3 is a homozygous viable and fertile, third chromosome insertion. In P{UAS-InR.del}, InR sequence deleted from Eco47III to XhoI to delete most of the alpha subunit was cloned into P{Express-UAS}. This construct mimics a construct expressing a constitutively active human InR described by Ellis et al., 1987 (Mol Endocrin. 1:15-24 ). P{UAS-InR.del}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-InR.del}3 is a homozygous viable and fertile, third chromosome insertion. In P{GMR-InR.del}, InR sequence deleted from Eco47III to XhoI to delete most of the alpha subunit was cloned into P{Express-glass}. This construct mimics a construct expressing a constitutively active human InR described by Ellis et al., 1987 (Mol Endocrin. 1:15-24 ). P{GMR-InR.del}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-InR.del}3 is a homozygous viable and fertile, third chromosome insertion. In P{UAS-InR.R418P}, InR sequences encoding a constitutively active R418P variant protein were cloned into P{Express-UAS}. This fly variant protein mimics the human K86P protein variant of Longo et al., 1993 (PNAS 90: 60-64). P{UAS-InR.R418P}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{UAS-InR.R418P}2 is a homozygous viable and fertile, second chromosome insertion. In P{GMR-InR.R418P}, InR sequences encoding a constitutively active R418P variant protein were cloned into P{Express-glass}. This fly variant protein mimics the human K86P protein variant of Longo et al., 1993 (PNAS 90: 60-64). P{GMR-InR.R418P}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-InR.R418P}3 is a third chromosome insertion. In P{UAS-InR.K1409A}, InR sequences encoding a dominant negative K1409A protein variant were cloned into P{Express-UAS}. P{UAS-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{UAS-InR.K1409A}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-InR.K1409A}3 is a homozygous viable and fertile, third chromosome insertion. In P{GMR-InR.K1409A}, InR sequences encoding a dominant negative K1409A protein variant were cloned into P{Express-glass}. P{GMR-InR.K1409A}1 is a homozygous and hemizygous viable and fertile, X chromosome insertion. P{GMR-InR.K1409A}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-InR.K1409A}3 is a homozygous viable and fertile, third chromosome insertion. In P{UAS-InR.A1325D}, InR sequences encoding a constitutively active A1325D variant protein were cloned into P{Express-UAS}. This fly variant protein mimics the human V938D protein variant of Longo et al., 1992 (J Biol. Chem. 267:12416-9 ). P{UAS-InR.A1325D}2 is a homozygous viable and fertile, second chromosome insertion. P{UAS-InR.A1325D}3 is a homozygous viable and fertile, third chromosome insertion. In P{GMR-InR.A1325D}, InR sequences encoding a constitutively active A1325D variant protein were cloned into P{Express-glass}. This fly variant protein mimics the human V938D protein variant of Longo et al., 1992 (J Biol. Chem. 267:12416-9 ). P{GMR-InR.A1325D}2 is a homozygous viable and fertile, second chromosome insertion. P{GMR-InR.A1325D}3 is a homozygous viable and fertile, third chromosome insertion. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX
