Date: Thu, 6 Feb 2003  16:43:50  \-0500 (EST)
From: Rolf Bodmer <rolf@XXXX>
Sender: <rolf@XXXX>
To: Chihiro Yamada <cy200@XXXX>
Subject: Re: FlyBase Query (cy1756)
...
Question 2:
EVEG92 is the one used to make the 86T, delta ES, and delta NS. The 86T
construct has a \+8.6kb endpoint, and both delta ES and NS have a \+9.2kb
endpoint. A wild type construct that has either endpoint gives the same
expression pattern.
thanks, rolf
On Wed, 5 Feb 2003, Chihiro Yamada wrote:
> Dear Dr Bodmer,
>
> I'm currently curating your paper for FlyBase:
>
> Han et al. 2002, Dev. Biol. 252(2) 225--240.
>
...
> tkv<up>1AΔGS.Scer\UAS. T:Ivir \HA1</up>
> P{UAS-tkv.1AΔGS}
> A dominant negative form of the tkv1 isoform, in which the GS
> box is deleted, tagged with HA1, is expressed under the control
> of Scer\UAS regulatory sequences.
>
> Could you tell me which one of these you used in your paper?
>
> eve rescue construct
> ====================
> In Fig 7 you describe 2 eve transgene constructs, that have been made by
> deleting portions of the eve mesoderm enhancer. These were made from a
> eve rescue construct for which you cite:
>
> Fujioka et al., 1999, Development 126(11)
>
> In this paper, 2 constructs were made that meet the description.
>
> P{E+L-eve.9.2.G} called EVEG92 in the Fujioka paper
> P{E+L-eve.9.2} called EVE92
>
> The only difference is the presence of w+mCG (a w+mC allele that
> has been modified by the addition of a gl activator sequence) in
> EVEG92.
>
> Can you tell me which one of these was used to make 86T insertion, and
> whether this was the same construct that was used as the basis for the
> P[eve+,emeΔES] and P[eve+,emeΔNS] constructs?
>
> Best wishes,
>
> Chihiro
>
> \----------------------------------------------------------------------
> Chihiro Yamada.
>
> FlyBase (Cambridge), http://fly.ebi.ac. uk:7081 /
> Department of Genetics,
> University of Cambridge, email: c.yamadagen.cam.ac.uk
> Downing Street, Tel : 01223-333963
> Cambridge, CB2 3EH, FAX : 01223-333992
> United Kingdom. Memes don't exist. Spread the Word.
> \----------------------------------------------------------------------
Dear Chihiro,
in the pers. communication, please, include Miki Fujioka, who made the T86
construct and related transgenic insertion lines. J48 and J43 are
insertion lines of the delta ES and delta NS construct, respectively
(+9.2kb endpoint). In fact, we tested another delta ES insertion line,
J49, which also abolished mesodermal expression, as J48.
I hope this clarifies the issue. thanks, rolf
On Fri, 7 Feb 2003, Chihiro Yamada wrote:
> Dear Rolf,
>
> Thanks for your prompt reply.
>
> > Question 2:
> > EVEG92 is the one used to make the 86T, delta ES, and delta NS. The 86T
> > construct has a \+8.6kb endpoint, and both delta ES and NS have a \+9.2kb
> > endpoint. A wild type construct that has either endpoint gives the same
> > expression pattern.
Dr. Rolf Bodmer Tel: 734-763-3182
Dept. of MCDB, NS3013 Fax: 734-647-0884
University of Michigan E-mail: rolf@XXXX
830 N University
Ann Arbor, MI 48109-1048
http://biology.lsa.umich.edu/research/labs/bodmer/index.htm