To: basler@XXXX
Subject: FlyBase query
From: Gillian Millburn (Genetics) <gm119@XXXX>
Date: Tue, 9 Jul 2002  10:41:22  \+0100
Dear Dr. Basler,
I am curating your paper for FlyBase:
Gerlitz and Basler, 2002, Genes Dev. 16(9): 1055--1059
and I have a few questions about some of the constructs and mutants you
used.
1. wf141 ( <up></up> = superscript).
You state that this mutant was described in:
Melendez et al., 1995, Genetics 141(4): 1507--1520
We have a record of a number of lethal mutations from that paper, but
none of the alleles have a symbol like '141'. Do you know which lethal
complementation group from that paper wf141 corresponds to (the
complementation groups are l(3)72Ab, l(3)72Ac, l(3)72Ad, l(3)72CDa,
l(3)72CDb, l(3)72CDc, l(3)72CDd, l(3)72CDe and l(3)72CDf) \- then I
could merge the lethal complementation group in with the wf gene in
FlyBase.
2. .. scalloped-Gal4
is this the 'SG29.1' line mentioned in :
Milan et al., 1997, Proc. Natl. Acad. Sci. USA 94(11): 5691--5696
thanks,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
Department of Genetics,
University of Cambridge,
Downing Street, email: gm119@XXXX
Cambridge, CB2 3EH, Ph : 01223-333963
UK. FAX: 01223-333992
\--------------------------------------------------------------
Date: Fri, 12 Jul 2002  22:04:32  \+0200
To: Gillian Millburn (Genetics) <gm119@XXXX>
From: Konrad Basler <basler@XXXX>
Subject: Re: FlyBase query
Dear Gillian,
here the answers to your questions:
>
>1. wf141 ( <up></up> = superscript).
>
>You state that this mutant was described in:
>
>Melendez et al., 1995, Genetics 141(4): 1507--1520
>
>We have a record of a number of lethal mutations from that paper, but
>none of the alleles have a symbol like '141'. Do you know which lethal
>complementation group from that paper wf141 corresponds to (the
>complementation groups are l(3)72Ab, l(3)72Ac, l(3)72Ad, l(3)72CDa,
>l(3)72CDb, l(3)72CDc, l(3)72CDd, l(3)72CDe and l(3)72CDf) \- then I
>could merge the lethal complementation group in with the wf gene in
>FlyBase.
wf141 is l(3)72CDf). We called it wf141 because of the protein
truncation at amino acid position 141.
>2. .. scalloped-Gal4
>
>is this the 'SG29.1' line mentioned in :
>
>Milan et al., 1997, Proc. Natl. Acad. Sci. USA 94(11): 5691--5696
No, this is S278, a Gal4 insertion line from our collection (see
Gerlitz, O., Nellen, D., Ottiger,M., and Basler K. 2002. A screen for
genes expressed in Drosophila imaginal discs. Int. J. Dev. Biol. 46:
173-176).
Please do not hesitate to contact me if there are further questions.
Thanks for your help!
Kind regards,
Konrad