From rd120@XXXX Mon Mar 18 09:52:28 2002 To: singh@XXXX Subject: Helping FlyBase: ADRC-10384 Dear Navjot, We are currently curating the abstracts for the upcoming 43rd (San Diego) Annual Drosophila Research Conference, for FlyBase. I am writing in connection with your abstract: 'Drosophila Sin3A-associated polypeptide p18 (dSAP18), a co-repressor that interacts with Bicoid and inhibits its activity.' You say 'To further study dSAP18 function, we generated dSAP18 mutants by remobilizing a P-element located 259bp away from dSAP18 start codon.' Is this a BDGP or other public P-element? If so would you be able to tell me which one it is? We have records for them all in FlyBase and we like to keep all the various data points correctly inter-related as much as possible. You mention a mutation called Delta-dSAP18-117 which deletes 2/3 of the coding sequence of Bin1 (dSAP18). Depending on whether this deletion also removes any part of any of the transcription unit I will make either an allele record (Bin1117) or an allele record (Bin1117) with a linked deletion chromosome record (Df(3R)Bin1117). Could you confirm for me that the deletion extends from the progenitor insertion to 2/3 of the way into the coding region of Bin1 only? Sorry to seem so particular \- but these questions do a make a difference to how we put the data into FlyBase and we like to get things as correct as possible at the point at which they go into FlyBase. Thank you very much for your help, with best wishes, Rachel. \---------------------------------------------------------------------- Rachel Drysdale, Ph.D. FlyBase (Cambridge), Department of Genetics, University of Cambridge, Downing Street, email: rd120@XXXX Cambridge, CB2 3EH, Ph : 01223-333963 UK. FAX: 01223-333992 FlyBase: http://fly.ebi.ac. uk:7081 / \---------------------------------------------------------------------- From singh@XXXX Wed Mar 20 19:27:21 2002 To: Rachel Drysdale (Genetics) <rd120@XXXX> Subject: Re: Helping FlyBase: ADRC-10384 Dear Rachel, In dSAP18 deletion screen, I used the P-element insertion line EP(3)3462 from BDGP database. This P-element is located 259 bp from start codon of dSAP18. The dSAP18 deletion I used in my abstract Bin1(117), is 601 bp deletion from p-element insertion site. So to be exact it removes 448bp out of 633bp dSAP18 gene. I hope it answers your questions. If not please do not hesitate to ask again. Thanks Navjot
