From kcook@XXXX Wed May 02  15:42:51  2001
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Luo constructs and insertions
The following information accompanied stocks donated to the Bloomington
Stock Center by Liqun Luo, Stanford University (5/01).
In the entry for P{UAS-Cdc42.S89} (FBtp0008218), two mutant Cdc42
constructs are described as synonymous, an 'S89' construct and an 'L89'
construct.
According to Liqun Luo:
>'These are in fact two different transgenes. The wild-type amino acid at
>89 position is Ser. Ser to Phe change (F89) was trying to mimic a
>dominant negative mutant in worm ras. L89 (Ser changes to Leu) was an
>error in making that intended F89 mutation. However we later found that
>L89 and F89 did have similar phenotypes when both transgenes are compared
>side-by-side. According to the convention S89 should be renamed F89. So
>we should keep L89 as it is and change S89 to F89.'
Insertions of these and other constructs from Luo include:
P{UAS-Cdc42.F89}3 third chromosome insertion
P{UAS-Cdc42.V12}2 third chromosome insertion
P{UAS-Cdc42.N17}3 second chromosome insertion
P{UAS-Cdc42.L89}4 third chromosome insertion
P{UAS-Drac1.L89}6 second chromosome insertion
P{UAS-Drac1.V12}1 third chromosome insertion
P{UAS-Drac1.N17}1 third chromosome insertion
P{UAS-Drac1.L}3 second chromosome insertion
All are homozygous viable and fertile insertions.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX