From Leonard.Rabinow@XXXX Wed Dec 20 09:21:50 2000 Envelope-to: gm119@XXXX Delivery-date: Wed, 20 Dec 2000 09:21:50 \+0000 X-Sender: rabino41@XXXX Mime-Version: 1.0 Content-Type: text/plain; charset='iso-8859-1' Content-Transfer-Encoding: quoted-printable Date: Wed, 20 Dec 2000 10:20:39 \+0100 To: Gillian Millburn (Genetics) <gm119@XXXX> From: Leonard Rabinow <Leonard.Rabinow@XXXX> Subject: Re: FlyBase query Content-Length: 4305 Hello Gillian, >I am curating your paper for FlyBase: >FBrf0130184 == Yun et al., 2000, Genetics 156(2): 749--761 >3. FlyBase has a record of a gene called 'Msu' ('Mosaic suppressor') >from the paper: > >Csink et al., 1994, Genetics 136(2): 573--583 > >In that paper they describe 2 alleles 'Msu' and 'Msu2' which they say >were originally isolated in mutagenesis screens described in: > >Birchler et al., 1989, Genes Dev. 3: 73--84 >Rabinow et al., 1991, Genetics 129: 463--480 > >In the Csink Genetics paper 'Msu' and 'Msu2' are said to be on 3rd >chromosomes with a number of breaks, including one at 98F and one near >centric heterochromatin in both cases. The 98F breakpoint is suggested >to be responsible for the mutant phenotype. They test for >complementation and say that Doa and Msu are discrete complementation >groups. > >I am writing to ask whether you know if the 'Msu' and 'Msu2' >mutations from the Csink Genetics paper are the same mutations as the >Doa alleles you call 'Msu' and 'Msu2' respectively in Table 2 of your >2000 Genetics paper. Yes, these are all one and the same. We have irrefutable evidence that Msu and Msu(2) are position-effected alleles of Doa. ... Csink and Birchler did not test as wide a range of alleles as we did, and there are several Doa-Msu (and Rem, yet another class- hence the Rem(gammaA)) alleles which do NOT complement. We examined all the Rem and Msu alleles for breaks in Doa using Southerns- there are none. However, some but not all of the Doa RNAs are strongly reduced in both Msu and Rem alleles, and the effects of both are suppressed by suppressors of PEV. If you care to cite this as a personal communication to Flybase, please feel free, but it should be cited as from Chi So and Leonard Rabinow (or however you abbreviate first names). I interpret the differences in allelic specificity reported by Csink and Birchler of Msu alleles on other TE-induced white alleles as almost certainly due to PEV effects on other loci nearby to Doa also affected in the chromsomal rearrangements. >I think that given the location of the genes, the overlap of authors on >the papers and the fact that in the Materials and Methods of your 2000 >Genetics paper you say that 'DoaMsu2 complements the vast majority of >other Doa mutations' that Msu = DoaMsu and Msu2 = DoaMsu2, but I >wanted to check before merging the two genes in FlyBase, See above. It would seem reasonable. >I look forward to hearing from you, I hope this was helpful. Please don't hesitate if you need any further information or clarification, or I can provide any help in any way. Best wishes, Lenny Rabinow \-------------------------------------------------------------------- Leonard Rabinow Laboratoire d'Embryologie Moléculaire B&acaret;timent 445 Université Paris XI 91405 ORSAY CEDEX FRANCE Leonard.Rabinow@XXXX Phone: 1-69-15-74-65 FAX: 1-69-15-68-02 (from US, prefix: 011-33)