>From sp691305@XXXX Wed Mar 11 17:06:06 1998 Subject: update for FlyBase: mm = mbl To: ag24@XXXX Dear Aubrey, I have another update for FlyBase. In the course of doing my molecular screen I have found that mindmelt (mm) (Kania et al., 1995) is allelic to muscleblind (mbl) (Begemann et al., 1997). I have plasmid rescued the genomic fragment flanking the 71/3 P element allele of mindmelt using EcoRI and sequenced both ends of the rescued fragment. The sequence of the proximal end of this fragment showed 98% identity (over 518 nt.) to the published sequence of the mbl cDNA (Begemann et al., 1997). In addition, the 71/3 allele of mm fails to partially complement (9/158) the strong mblE27 allele (P element excision which affects the coding region of the gene and is presumably a null allele (Begemann et al., 1997)). Escapers had wing blisters and wing venation defects; two flies had unexpanded wings. The 71/3 allele of mm complemented two weak alleles of mbl - mbl05507 (P element insertion allele) (26/97) and mblE127 (P element excision which does not affect the coding region of the gene) (42/155). Finally, the 71/3 mindmelt insertion was mapped by in situ hybridization to 54A (Kania et al., 1995) and the muscleblind gene was mapped to 54A1-3 (Begemann et al., 1997). Therefore, I concluded that mm is allelic to mbl. This information, when included in FlyBase, should be referred to as S.Prokopenko and H.J.Bellen, personal communication. Feel free to contact me at sp691305@XXXX, if you have any questions or need more detailed information. Best wishes, Sergei Sergei Prokopenko Bellen lab
