From bhochman@XXXX Mon Jul 28 18:40:44 1997 Envelope-to: rd120@XXXX Delivery-date: Mon, 28 Jul 1997 18:40:44 +0100 Date: Mon, 28 Jul 1997 13:45:40 -0400 (EDT) X-Sender: bhochman@XXXX (Unverified) X-Mailer: Windows Eudora Light Version 1.5.2 Mime-Version: 1.0 Content-Type: text/plain; charset='us-ascii' To: rd120@XXXX From: Benjamin Hochman <bhochman@XXXX> Subject: FlyBase Inquiry Content-Length: 1895 Dear Rachel, I found an old stocklist ( from 1968 ) that may clear things up a bit. When we first got chromosome 4 lethals ( found by our lab or sent by others ) they were given designations and then tested for allelism ( inter se and with lethal stocks already present). Those found in a 1963 Knoxville orchard were labeled K-1 to K-7, and those sent by Alice Kenyon were labeled AK-39, etc. After allelism tests were finished, the lethal designations were changed. The table below gives the correct symbols and their former designations. CORRECT NOMENCLATURE FORMER DESIGNATION l(4)1e K-7 1(4)2h K-5 l(4)16 K-4 l(4)20 K-3 l(4)29b K-1 l(4)1f AK-376 l(4)5a AK-354 l(4)10a AK-209 l(4)16b AK-39 l(4)16c AK-60 l(4)33c AK-82 Unfortunately, I could find no K-2 or K-6. But at least this gives some evidence as to the possible correspondence of K-1 and K-3 to stocks you already have. As to the stocks you call 'Kenyon' lethals, some corrections are in order. 16a was not found by Kenyon. Nor were 10 and 33. She found 10a and 33c, as well as 1f, 5a, 16b and 16c. You could run allelism tests of the lethal you presently call K-2 against the other chromosome 4 stocks in your collection. I assume all of these lethals are still balanced over ciD. If so, just collect a bunch of virgin females from the K-2 stock and cross them separately to males from the other stocks. If non-ciD progeny appear, the lethals are non-allelic. I hope this helps you and Kathy Matthews. Best wishes, Ben