From kedwards@XXXX Thu Jun 19  00:04:31  1997
Date: 	Wed, 18 Jun 1997  18:02:54  -1000
From: Kevin A Edwards <kedwards@XXXX>
X-Sender: kedwards@uhunix3
To: Madeline Crosby <crosby@XXXX>
Subject: Re: CaSpeR-hs
Mime-Version: 1.0
Dear Lynn,
Here is a sequence run off a GMR vector. The specific insert, a Src29A
cDNA, was cloned after the RI site and is deleted here, so this sequence
should be common to all GMRs. The primer was made to Casper-hs sequence
upstream of the Xho site, pointing forward into the promoter. The sequence
begins with a match to casper-hs up to the Xho site, about base 20. Then
the glass pentamer begins after the Xho- all 5 repeats are perfect. This
goes into a Sal site and polylinker (about base 200), and then the
promoter sequence. It ends with the PCR primer B. Hay lists in
Dev. 120:2121 , terminating in the EcoRI cloning site. Presumably the rest
of GMR is identical to casper-hs. The sequence is SS but unambiguous. Hope
it helps! If you have any trouble putting the whole plasmid together based
on this let me know.
GMR-Src29A promoter region, 497 bp:
TTTGGCAGAAAGAAAAcTCGAGCCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAG
CCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAGCCCAGTGGAAACCCTTGAAATG
CCTTTAAGTCGAGCCCAGTGGAAACCCTTGAAATGCCTTTAAGTCGAGCCCAGTGGA
AACCCTTGAAATGCCTTTAAGTCGACTCTAGAGGATCCGCGGCCGCGGGTACCGGGG
GATCCGGCGAAAAGAGCGCCGGAGTATAAATAGAGGCGCTTCGTCTACGGAGCGACA
ATTCAATTCAAACAAGCAAAGTGAACACGTCGCTAAGCGAAAGCTAAGCAAATAAAC
AAGCGCAGCTGAACAAGCTAAACAATCTGCAGTAAAGTGCAAGTTAAAGTGAATCAA
TTAAAAGTAACCAGCAACCAAGTAAATCAACTGCAACTACTGAAATCTGCCAAGAAG
TAATTATTGAATACAAGAAGAGAACTCTGAATAGGGAATTC
Kevin Edwards
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
kedwards@XXXX Yamamoto Behavior Genes Project
fax 808-956-2440 CCRT, University of Hawaii
lab 808-956-4909 Honolulu HI 96822