FAX: 00-1-206-667-6522 Dear Michael, I have a question about an allele of fog you used in your Cell paper: \*x FBrf0068458 == Costa et al., 1994, Cell 76(6): 1075--1089 You call it fog[RA67]. Is it possibly the same chromosome as Df(1)RA67, from the Umea stock list? >U-#18350 Df(1)RA67 / FM7, y[31d] sc[8] w[a] sn[X2] v[Of] g[4] B[1] / y[+] > Ymal[106] >Breakpts: ?; 20B-D >Comments: >Center: Umea Stock Center Contact: Karin Ekstrom karin.ekstrom@XXXX Many thanks for your help, best wishes, Rachel. From mcosta@XXXX Mon Oct 30 23:01:50 1995 Date: Mon, 30 Oct 95 14:56:28 PST Mime-Version: 1.0 Content-Type: text/plain; charset='us-ascii' To: rd120@XXXX From: mcosta@XXXX Subject: fog[RA67] Content-Length: 1631 Dear Rachel, The RA67 allele of fog mentioned in my Cell paper is at least derived from the the stock called Df(1)RA67 and quite possibly identical to it. When I tested the Df(1)RA67 strain that Eric Wieschaus had in the lab, I found that it complemented all mutations tested on both the proximal and distal side of fog. Therefore, I started calling it fog[RA67] since there was no genetic evidence that our allele was a deficiency. I never checked cytologically for a deletion in this strain and never found any molecular evidence of a deletion. Len Kelly has also found that his stock labelled Df(1)RA67 also behaves genetically as a simple fog mutation, and it is not clear whether Eric got his allele from Len or the Umea stock center. In any case, either the original complementation tests were done wrong for this strain (as I suspect), or the strain that Eric and Len have has changed from a deficieny to a fog allele over time. Obviously, if you plan to use this strain you should do the complementation tests on the stock you have. Feel free to send an e-mail message if you need further info. Mike Mike Costa 1100 Fairview Ave. N. Fred Hutchinson Cancer Research Center Dept. of Basic Sciences, A3-013 Seattle, WA 98109 USA phone:(206) 667-4085 FAX: (206) 667-3650
