From gelbart@XXXX Thu Mar 23 13:25:44 1995 Date: Thu, 23 Mar 95 08:26:21 EST From: gelbart@XXXX (William Gelbart) To: rd120@XXXX Subject: Re: and now for something completely different Cc: flybase-updatesXXXX, gelbartXXXX, padgett@XXXX Content-Length: 934 Hi Rachel, In(2LR)CyO-P20 is a CyO derivative in which a 20 kb transposon from ca. 72 to ca. 92 on the molecular map of dpp (St. Johnston et al., 1990) that covers all functions mediated by the dpp[Hin] and dpp[shv] region is inserted into CyO (polytene insertion position unknown). The 20 kb fragment is from a SalI digest of genomic DNA (I think from our dpp[+] dp cn bw isogenic strain that was also used as the RNA source for Nick Brown's cDNA libraries). In(2LR)CyO-P20 completely suffices for dpp function, even in the absence of any other copies of dpp in the genome. (In other words, it acts as if it is disomic for the dpp[Hin+] function.) This construct was made by Rick Padgett while he was a postdoc in my laboratory. I would like this personal communication to be cited as Padgett and Gelbart, pers. comm. to FlyBase. I am cc'ing this message to Rick in case he wishes to add to or amend anything I wrote. Bill