Twenty female larvae were collected at the wandering L3 stage (108-117 h AEL at 25oC) and bled in Schneider medium complemented with N-phenylthiourea on ice. Both circulating and resident pools of hemocytes were collected by first puncturing the cuticle to release the circulating hemocytes, then by scrapping the cuticle with fine forceps to release the resident hemocytes (FBrf0230406). After bleeding, the hemolymph was filtered on a 100um-mesh to remove cell aggregates and wasp eggs.