FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Bulli, Mon1
FlyBase ID
FBig0000120442
Interaction Network
Interactions Browser links
Bulli network
Mon1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0245700-2.CH.MW
Reference
Langemeyer et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mon1 

Mon1

neutral component
Mon1 

Mon1

neutral component
Mon1 

Mon1

neutral component
Bulli 

CG8270

neutral component
Bulli 

CG8270

neutral component
Bulli 

CG8270

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Trimeric complex containing Mon1, Ccz1 and CG8270 captured as a series of three binary interactions.

(Langemeyer et al., 2020)

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and Sf21 cell system.

(Langemeyer et al., 2020)
FBrf0246185-1.coIP.MS
Reference
Dehnen et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Bulli 

Bulli

bait

GFP tag

Mon1 

Mon1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Dehnen et al., 2020)
FBrf0246185-4.PD.MW
Reference
Dehnen et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mon1 

Mon1

bait

GST tag

Bulli 

Bulli

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

(Dehnen et al., 2020)
FBrf0246185-6.CH.MW
Reference
Dehnen et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mon1 

Mon1

neutral component
Mon1 

Mon1

neutral component
Mon1 

Mon1

neutral component
Bulli 

Bulli

neutral component
Bulli 

Bulli

neutral component
Bulli 

Bulli

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and Sf21 cell system.

(Dehnen et al., 2020)

Mon1,Ccz1 and Bulli can form a trimer in solution; captured as three separate binary interactions.

(Dehnen et al., 2020)
FBrf0256532-2.PD.CH.EM
Reference
Yong et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mon1 

Mon1

neutral component

His tag

Mon1 

Mon1

neutral component

His tag

Mon1 

Mon1

neutral component

His tag

Bulli 

Bulli

neutral component

FLAG tag

Bulli 

Bulli

neutral component

FLAG tag

Bulli 

Bulli

neutral component

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mon1-Ccz1-Bulli trimer captured as three pairwise interactions.

(Yong et al., 2023)

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

(Yong et al., 2023)
FBrf0257015-1.PD.CH.MW
Reference
Borchers et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mon1 

Mon1

bait

GST tag

Bulli 

Bulli

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in baculovirus system.

(Borchers et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (4)