FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000114639
Interaction Network
Interactions Browser links
mir-310 network
Dg network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226267-11a.miR-mRNA.IHC
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-310

bait
Dg 

Dg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female follicle cells of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

Overexpression of mir-310, mir-311, mir-312 and mir-313 in ovarian follicle cell clones reduces Dg protein levels.

FBrf0226267-2a.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-310

bait
Dg 

Dg

prey
Dg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dg 
long 3-prime UTR
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Luciferase reporter assay.

FBrf0226267-7a.miR-mRNA.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-310

bait
Dg 

Dg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Deletion of mir-310, mir-311, mir-312 and mir-313 (using Df(2R)KT40) results in increased levels of Dg mRNA, particularly the long 3\'UTR isoforms.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)