FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cp110, Klp10A
FlyBase ID
FBig0000109476
Interaction Network
Interactions Browser links
Cp110 network
Klp10A network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0217842-1.PD.A
Reference
Delgehyr et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Klp10A 

Klp10A

bait

GST tag

Cp110 

Cp110

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Delgehyr et al., 2012)

Interaction observed with both isoforms of Cp110.

(Delgehyr et al., 2012)
FBrf0217842-2.PD.WB
Reference
Delgehyr et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp110 

Cp110

bait

GST tag

Klp10A 

Klp10A

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Delgehyr et al., 2012)

Interaction observed with both isoforms of Cp110.

(Delgehyr et al., 2012)
FBrf0217842-3.coIP.WB
Reference
Delgehyr et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
Cp110 

Cp110

bait

GFP tag

Klp10A 

Klp10A

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction observed with both isoforms of Cp110.

(Delgehyr et al., 2012)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Delgehyr et al., 2012)
FBrf0217842-4.PD.A
Reference
Delgehyr et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cp110 

Cp110

bait

GST tag

Klp10A 

Klp10A

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Delgehyr et al., 2012)
FBrf0257757-7.coIP.WB
Reference
Ryniawec et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cp110 

Cp110

bait

GFP tag

Klp10A 

Klp10A

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ryniawec et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)