FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dco, per
FlyBase ID
FBig0000105532
Interaction Network
Interactions Browser links
dco network
per network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0137069-1.coIP.WB
Reference
Kloss et al., 2001
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait
per 

PER

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Kloss et al., 2001)
FBrf0167898-1.PD.A
Reference
Preuss et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

dco 
per 

PER

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dco 
N-terminal domain
sufficient binding region
aa 1-292
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Preuss et al., 2004)
FBrf0167898-2.coIP.WB
Reference
Preuss et al., 2004
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait
dco 
dco 
per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dco 
N-terminal domain residue 47
mutation decreasing interaction
aa 47

P47S, coordinates relative to dco-PA

dco 
N-terminal domain residue 80
mutation decreasing interaction
aa 80

M80I, coordinates relative to dco-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Preuss et al., 2004)
FBrf0200748-1.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
dco 

dbt

bait

V5 tag

per 

dPER

prey
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
DBT binding domain
necessary binding region
aa 749-804

"dPERΔ" construct, coordinates relative to per-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim et al., 2007)
FBrf0200748-4.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

bait

HA tag

per 
dco 

DBT

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
DBT binding domain
necessary binding region
aa 749-804

"dPERΔ" construct, coordinates relative to per-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kim et al., 2007)
FBrf0203042-1.PD.A
Reference
Muskus et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

per 

PER

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Catalytically inactive dco (K38R) can still bind per.

(Muskus et al., 2007)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Muskus et al., 2007)
FBrf0203042-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

enzyme

Myc tag

dco 

DBT

enzyme

Myc tag

dco 
per 

PER

enzyme target

His tag, P[32] label

per 

PER

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0205742-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

dbt

enzyme

GST tag

dco 
dco 

dbt

enzyme

GST tag

per 

per

enzyme target

GST tag, P[32] label

per 
per 

per

enzyme target

GST tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0205742-2.ENZ.MS
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

dbt

enzyme

GST tag

dco 

dbt

enzyme

GST tag

per 

per

enzyme target

GST tag, P[32] label

per 
per 

per

enzyme target

GST tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0207013-2.coIP.WB
Reference
Yu et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait
per 

PER

prey
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain
necessary binding region
aa 755-809
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yu et al., 2009)

dco coimmunoprecipitates per only when per is hyperphosphorylated.

(Yu et al., 2009)
FBrf0207159-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

enzyme

Myc tag

dco 

DBT

enzyme

Myc tag

dco 
dco 
dco 
per 

PER

enzyme target

His tag, P[32] label

per 

PER

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0212171-1.coIP.WB
Reference
Sun et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dco 

dco

bait

V5 tag

per 

per

prey
per 
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
conserved region 3
mutation decreasing interaction
aa 768-842
per 
PER DBT binding domain (PDBD)
mutation decreasing interaction
aa 755-809
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2010)
FBrf0214559-1.coIP.WB
Reference
Syed et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

V5 tag

dco 

dco

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Syed et al., 2011)
FBrf0228758-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

enzyme

Myc tag

dco 

DBT

enzyme

Myc tag

per 

PER

enzyme target

His tag, P[32] label

per 

PER

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0233199-1.PD.WB
Reference
Fu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Fu et al., 2016)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult head extract.

(Fu et al., 2016)
FBrf0240942-3.PD.WB
Reference
Lam et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

per 

PER

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cells.

(Lam et al., 2018)

The dco-per interaction is increased with coexpression of CkIα.

(Lam et al., 2018)
FBrf0240942-4.PD.WB
Reference
Lam et al., 2018
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dco 

DBT

bait

GST tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult heads.

(Lam et al., 2018)

The dco-per interaction is decreased with knock-down of CkIα.

(Lam et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (12)