Interaction in vitro; bait derived from wild-type embryonic extract; prey produced as a recombinant fusion protein in bacterial system.

Source was embryonic glycoprotein extract of wild-type fly line; prey produced as a recombinant protein; bait produced from endogenous gene.

Lyophilized recombinant trol was added to embryonic glycoprotein extract. Extract with trol was then precipitated with an antibody to Dg and visualized by western blot with an antibody against trol.