Overexpression of mir-9a reduces Dg protein levels.

Source was follicle cells of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

luciferase reporter assay

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Mutation of mir-9a increases Dg mRNA levels.

Source was adults of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

mir-9a was expressed post-developmentally to avoid muscle loss and degeneration that could confound the analysis.

Overexpression of mir-9a decreases Dg mRNA levels.

Source was adults of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.