Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

asl self-interacts via specific domain interactions. asl-B and asl-C self-interact. asl-B and asl-C interact strongly while asl-C weakly interacts with asl-A. No information is available for asl-A self-interactions and no strong result is observed for asl-A with asl-B.

SEC-MALS analysis, asl fragments oligomerize.

Interaction in vitro; substrate produced as a protein fragment in bacterial system.

asl self-interacts.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The central and C-terminal regions of asl tested homo- and heterodimerize in this assay. Weak interactions with the N-terminal region of asl were also observed.

Estimated molecular weight is consistent with a dimer.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.