Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system.

Treatment with okadaic acid, a phosphatase inhibitor, disrupts the interaction, while mutation of gnu phosphorylation sites restores the interaction, suggesting that phosphorylated gnu does not interact with png.

Source was oocytes of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

Mutation of gnu phosphorylation sites allows interaction with png in stage 14 oocytes when normally no interaction is detected at this stage.