FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102608
Interaction Network
Interactions Browser links
SAK network
SkpA network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212014-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SAK 

Plk4

bait

Protein A tag

SkpA 

SkpA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0225974-3.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SAK 

PLK4

bait

Protein A tag

SAK 
SkpA 

SkpA

prey

1 unique peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SAK 
kinase domain
mutation disrupting interaction
aa 22aa 43aa 146

K22M, one of three mutations in the kinase dead (KD) mutant

K43M, one of three mutations in the kinase dead (KD) mutant

D146A, one of three mutations in the kinase dead (KD) mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Kinase dead SAK does not interact with SkpA.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)