Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

The da-l(1)sc interaction is stronger than da-ac, which is stronger than da-sc.

A [32]P labeled DNA probe consisting of an E box was mixed with sc and/or da. Cooperative binding to the DNA was observed by EMSA.

da and sc bind cooperatively to DNA. For the DNA probe tested, neither da nor sc could bind alone.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

A [32]P labeled DNA probe consisting of an E box from a ase regulatory fragment was was mixed with da and/or sc. Cooperative binding to the DNA was observed by EMSA.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Two-hybrid system: yeast, using a lacZ reporter under the control of hb regulatory sequence.

The hb reporter construct responds more strongly to the da-sc combination than to the da-l(1)sc combination, or to da alone. sc or l(1)sc alone do not have activity.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

Bait endogenous to embryonic extract.

Prey endogenous to embryonic extract.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

sc interacts with da but not with sisA or dpn.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from genomic expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

Two-hybrid system: yeast LexA-BD/B42-AD