FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hry, DCTN1-p150
FlyBase ID
FBig0000099069
Interaction Network
Interactions Browser links
hry network
DCTN1-p150 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0222317-16a.PD.MS
Reference
Dix et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

hairy

bait

TRAP tag

hry 
hry 
DCTN1-p150 

p150[Glued]

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
HLE
sufficient binding region
CAUUUCACAUCAUUCGCCGGGAUUGCGCAAAUGUUGCUUUGAAGUGUUGCAAACAUGCGAAUCCUAAACUCGGUUCACAACUUCGUUGGCUUAGUUUCCUGGCUUAUAUCCUGGAAACCCGUCGAC
hry 
HLE-SL1
mutation disrupting interaction
nt 41-43

GAA-to-CUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Dix et al., 2013)

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).

(Dix et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)