FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Chrac-16, Acf
FlyBase ID
FBig0000098834
Interaction Network
Interactions Browser links
Chrac-16 network
Acf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0097684-3.CH.MS
Reference
Varga-Weisz et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 15embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Acf 

175K subunit

neutral component
Acf 

175K subunit

neutral component
Acf 

175K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.

(Varga-Weisz et al., 1997)

Iswi, Acf1, Chrac-14 and Chrac-16 interact as part of the approximately 670 kDa CHRAC complex.

(Varga-Weisz et al., 1997)

Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0129776, FBrf0138380).

(Varga-Weisz et al., 1997)

Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).

(Varga-Weisz et al., 1997)

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Varga-Weisz et al., 1997)
FBrf0138380-13.coIP.WB
Reference
Eberharter et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 15embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chrac-16 

Chrac16

bait
Acf 

Acf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Eberharter et al., 2001)

Antibody used in immunoprecipitation raised against a Chrac-14/Chrac-16 recombinant heterodimer.

(Eberharter et al., 2001)
FBrf0138380-2.CH.WB
Reference
Eberharter et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 15embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Acf 

175K subunit

neutral component
Acf 

175K subunit

neutral component
Acf 

175K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Chrac-16 

20K subunit

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.

(Eberharter et al., 2001)

Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.

(Eberharter et al., 2001)

Iswi, Acf1, Chrac-14 and Chrac-16 interact as part of the approximately 660 kDa CHRAC complex.

(Eberharter et al., 2001)

Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0097684, FBrf0129776) and confirmed by western blot.

(Eberharter et al., 2001)

Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).

(Eberharter et al., 2001)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)