Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.
Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0129776, FBrf0138380).
Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Identity of CHRAC subunits determined in this and other publications by mass spectrometry (FBrf0097684, FBrf0129776) and confirmed by western blot.
Top2 later shown to be a contaminant that is not part of the CHRAC complex (FBrf0138380).
Source was embryonic nuclear extract of wild-type fly line; proteins produced from endogenous genes.
Nuclear extract was fractionated through several different chromatographic steps, selecting for chromatin remodeling activity.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.