FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
per, tim
FlyBase ID
FBig0000098280
Interaction Network
Interactions Browser links
per network
tim network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0109474-4.Y2H
Reference
Ceriani et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

fused to LexA DNA-binding domain

tim 

TIM

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ceriani et al., 1999)

The per-tim interaction was observed in light and in dark conditions.

(Ceriani et al., 1999)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Ceriani et al., 1999)
FBrf0110163-2.coIP.A
Reference
Lee et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

[35]S label

tim 

TIM

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Lee et al., 1999)
FBrf0137069-2.coIP.WB
Reference
Kloss et al., 2001
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

HA tag

tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kloss et al., 2001)
FBrf0137204-3.Y2H
Reference
Rosato et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait

fused to LexA DNA-binding domain

tim 
per 

PER

prey

fused to B42 activation domain

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain
sufficient binding region
aa 233-685

coordinates relative to per-PA

tim 
interaction domain
sufficient binding region
aa 377-915

coordinates relative to tim-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Rosato et al., 2001)

Two-hybrid system: yeast LexA-BD/B42-AD

(Rosato et al., 2001)
FBrf0200748-2.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
tim 

tim

bait

HA tag

per 

dPER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim et al., 2007)
FBrf0200748-6.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

bait
tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kim et al., 2007)
FBrf0207795-3.Y2H
Reference
Hennig et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tim 

dTIM

bait

fused to lexA DNA-binding domain

per 

dPER

prey

fused to B42 activation domain

per 
per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
PAS domains
sufficient binding region
aa 232-538
per 
PAS B domain
mutation disrupting interaction
aa 474,492,494

E474R,H492S,R494D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hennig et al., 2009)

Two-hybrid system: yeast lexA-BD/B42-AD

(Hennig et al., 2009)
FBrf0207796-2.coIP.WB
Reference
Landskron et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

Myc tag

tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Landskron et al., 2009)
FBrf0210011-5.coIP.WB
Reference
Menet et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait
tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Menet et al., 2010)
FBrf0212171-3.coIP.WB
Reference
Sun et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tim 

tim

bait

HA tag

per 

per

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2010)
FBrf0214253-1.coIP.WB
Reference
Hara et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

HA tag

tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hara et al., 2011)

The tim P115L mutation does not affect interaction with per.

(Hara et al., 2011)
FBrf0214282-1.coIP.WB
Reference
Saez et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

Myc tag

tim 

tim

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Saez et al., 2011)
FBrf0221205-1.Y2H
Reference
Ozturk et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait

fused to LexA DNA-binding domain

per 

PER

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ozturk et al., 2013)

Two-hybrid system: yeast LexA-BD/B42-AD

(Ozturk et al., 2013)

Positive control.

(Ozturk et al., 2013)
FBrf0221794-1.coIP.WB
Reference
Li and Rosbash, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

V5 tag

tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li and Rosbash, 2013)

tim binds wild type per and the mutant pers proteins equally well.

(Li and Rosbash, 2013)
FBrf0231164-1.coIP.WB
Reference
Yildirim et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait
tim 

TIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Yildirim et al., 2015)

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Yildirim et al., 2015)
FBrf0232849-1.coIP.WB
Reference
Top et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait

HA tag

per 

PER

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Top et al., 2016)

Positive control.

(Top et al., 2016)
FBrf0232849-3.coIP.WB
Reference
Top et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

Myc tag

tim 

TIM

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Top et al., 2016)

Positive control.

(Top et al., 2016)
FBrf0232849-7.coIP.WB
Reference
Top et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait
per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Top et al., 2016)

Positive control.

(Top et al., 2016)
FBrf0244295-1.coIP.WB
Reference
Martin Anduaga et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2c1
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

V5 tag

tim 

TIM

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction with per was shown for tim isoforms TIM-L and TIM-SC.

(Martin Anduaga et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Martin Anduaga et al., 2019)
FBrf0244295-2.coIP.WB
Reference
Martin Anduaga et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2c1
Participants
Corresponds to
Reported as
Role
Note
tim 

TIM

bait

HA tag

per 

PER

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction with per was shown for tim isoforms TIM-L and TIM-SC.

(Martin Anduaga et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Martin Anduaga et al., 2019)
FBrf0255496-1.coIP.WB
Reference
Giesecke et al., 2023
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

V5 tag

tim 

Tim

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Giesecke et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (17)