FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sas-4, Grip128
FlyBase ID
FBig0000097871
Interaction Network
Interactions Browser links
Sas-4 network
Grip128 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0216370-21.coIP.MS
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Grip128 

Grip128

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2011)
FBrf0216370-22.coIP.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Grip128 

Grip128

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2011)
FBrf0219801-24.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Grip128 

Grip128

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

Grip128 coimmunoprecipitates and co-fractionates with Sas-4 as part of the S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)
FBrf0219801-46.PD.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait

GST tag

Sas-4 
Grip128 

Grip128

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sas-4 
N-terminal region
sufficient binding region
aa 1-190
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Gopalakrishnan et al., 2012)
FBrf0219801-5.coIP.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sas-4 

Sas-4

bait
Grip128 

Grip128

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gopalakrishnan et al., 2012)

Sas-4 interacts with Grip128 as part of the S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

The interaction of Sas-4 with Grip128 is disrupted by the addition of a non-hydrolyzable GTP analog, GMPCPP, to the extract.

(Gopalakrishnan et al., 2012)

The interaction of Sas-4 with Grip128 is increased by the addition of griseofulvin to the extract, a compound that induces hydrolysis of tubulin-bound GTP to GDP.

(Gopalakrishnan et al., 2012)

Coimmunoprecipitation from "high-speed lysates" depleted of mature centrosomes.

(Gopalakrishnan et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)